Product Description

OptiPrep™ is a sterile, endotoxin-tested density gradient medium composed of 60% (w/v) iodixanol in water. It is designed for high-resolution separation and purification of biological particles using density gradient centrifugation.

Due to its non-ionic nature, low viscosity and iso-osmotic properties, OptiPrep™ provides gentle separation conditions suitable for sensitive biological samples including cells, viruses, subcellular organelles, membranes, extracellular vesicles and macromolecules.

OptiPrep™ is widely used in molecular biology, virology, cell biology and biomedical research laboratories requiring reliable density-based fractionation workflows.

Specifications

Applications

Cell Isolation

OptiPrep™ enables gentle density-based separation of cellular populations while maintaining sample integrity.

• Primary cell isolation
• Cell enrichment
• Cell fractionation

Virus Purification

Suitable for purification and analysis of viral particles and viral vectors.

• Virus concentration
• Viral particle purification
• Viral vector research
• AAV purification workflows

Subcellular Organelle Isolation

OptiPrep™ gradients can be used for separation of cellular compartments and membrane fractions.

• Mitochondria
• Nuclei
• Membrane fractions
• Vesicles
• Subcellular compartments

Extracellular Vesicles & Macromolecules

• Exosomes
• Extracellular vesicles
• Lipoproteins
• Protein complexes

Sample Types

• Mammalian cells
• Viral particles
• Viral vectors
• Extracellular vesicles
• Organelles
• Membranes
• Macromolecular complexes

Features & Benefits

• Ready-to-use iodixanol density gradient medium
• Sterile formulation
• Endotoxin-tested
• Non-ionic and metabolically inert
• Low viscosity for efficient fractionation
• Iso-osmotic gradients for sensitive biological samples
• Suitable for viruses, organelles, membranes and cells
• Compatible with ultracentrifugation workflows

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