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MOLECULAR BIOLOGY • qPCR TROUBLESHOOTING What Causes High Ct Values in qPCR? Understanding the causes of delayed amplification and how to improve quantitative PCR results. Quantitative polymerase chain reaction (qPCR) is widely used to detect and quantify specific DNA sequences. In reverse transcription qPCR (RT-qPCR), RNA is first converted into complementary DNA (cDNA) before amplification. One important parameter in these experiments is the cycle threshold (Ct), also called the quan …

MICROBIOLOGY • HUMAN HEALTH • SCIENTIFIC RESEARCH The Human Microbiome: Understanding the Invisible Ecosystem Inside and Around Us Explore the microorganisms that inhabit the human body, their biological functions, their relationship with health, and the laboratory technologies advancing microbiome research. What Is the Human Microbiome? The human microbiome refers to the complex collection of microorganisms, their genetic material, and their biological activities associated with …

MICROBIOLOGY • VIROLOGY • BIOTECHNOLOGY Bacteriophages:  Infection Mechanisms and Therapeutic Applications Understanding the viruses that infect bacteria and their emerging role in antimicrobial research, biotechnology and modern medicine. Bacteriophage structure, infection mechanisms and scientific applications. 1. Introduction to Bacteriophages Bacteriophages, commonly known as phages, are viruses that specifically infect bacterial cells. They are among the most abundant …

PROTEIN PURIFICATION | MOLECULAR BIOLOGY Ni-NTA Magnetic Agarose Beads: Principle, Protocol and Applications Understanding magnetic affinity purification of His-tagged recombinant proteins, from molecular interactions to laboratory applications. Ni-NTA Magnetic Agarose Beads are affinity purification materials designed for the isolation of recombinant proteins containing polyhistidine tags (His-tags). They combine nickel-based affinity chemistry with magnetic separation technology to simplif …

IMMUNOASSAYS & ELISA KITS Human PTX3/Pentraxin 3 ELISA Kit PicoKine® Sensitive quantitative measurement of human Pentraxin 3 in biological samples. Product Information CAT# 0519-EK0861 Size 96 Well With Removable Strips Target Human PTX3 / Pentraxin 3 Sample Types Cell culture supernatants, serum, EDTA plasma and saliva Sensitivity 10 pg/mL Dry Ice No Hazardous No Login for Price 24/48H Stock Items & 2 to 6 Weeks non Stock Items. ADD TO CART SEND REQUEST …