HPLC Column: Topsil Silica, 5 μm, 4.6 × 250 mm

HPLC & CHROMATOGRAPHY

HPLC Column: Topsil Silica, 5 μm, 4.6 × 250 mm

Silica HPLC column designed for efficient separation of polar and lipophilic compounds.

HPLC Column Topsil Silica 5 μm 4.6 x 250 mm

Product Information

CAT#	1686-00450-01043
Size	5 μm, 4.6 × 250 mm
Particle Size	5 μm
Column Diameter	4.6 mm
Column Length	250 mm
Pore Size	150 Å
Bonding Phase	Top-Silica
Brand	Topsil
Dry Ice	No
Hazardous	No

Availability:
24/48H Stock Items & 2 to 6 Weeks non Stock Items.

Description

Topsil Silica is an HPLC column designed for the separation of highly polar and lipophilic compounds. Its silica-based stationary phase provides distinct selectivity compared with other silica column configurations.

The column combines lower operating pressure with high separation efficiency, making it an economical option for routine HPLC applications.

The 5 μm particle size and 4.6 × 250 mm column dimensions provide a practical format for analytical HPLC separations.

Technical Specifications

Parameter	Specification
Brand	Topsil
Particle Size	5 μm
Column Dimensions	4.6 × 250 mm
Internal Diameter	4.6 mm
Length	250 mm
Pore Size	150 Å
Silica Purity	99.99%
Surface Area	260 m²/g
Bonding Phase	Top-Silica

Features & Benefits

High-Purity Silica Uses high-purity silica with reported 99.99% purity, a 150 Å pore size, and a 260 m²/g surface area.	Efficient Separation Designed to provide efficient HPLC separation of highly polar and lipophilic compounds.
Lower Back Pressure The column is designed to operate with lower back pressure while maintaining high separation efficiency.	Good Peak Shape The endcapped stationary phase is designed to support good peak shape and extended column lifetime.
Suitable for Small Molecules Suitable for analytical separations involving small molecules and peptides.	Broad Phase Selection The Topsil range includes C18, C8, Phenyl-Hexyl, Silica, NH2, HILIC NH2 and CN phases.

HPLC Applications

Separation of highly polar compounds
Separation of lipophilic compounds
Small molecule analysis
Peptide analysis
Routine analytical HPLC
Research and laboratory chromatography

Available Topsil Phases

The Topsil column range includes multiple stationary phases for different chromatographic applications:

Phase	Application Focus
C18	Reversed-phase chromatography
C8	Reversed-phase chromatography
Phenyl-Hexyl	Aromatic and hydrophobic compound separations
Silica	Polar compound separations
NH2	Polar and carbohydrate-related separations
HILIC NH2	Hydrophilic interaction chromatography
CN	Alternative selectivity for analytical separations

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