Ni NTA Beads

PROTEIN PURIFICATION

Ni NTA Beads

Nickel-NTA agarose beads for purification of 6xHis-tagged proteins.

Ni NTA Beads

Product Information

CAT# 1637-SA004100
Size 100 mL
Bead Diameter 90 µm
Matrix 4% Agarose
Ligand Nitrilotriacetic Acid (NTA)
Charged Ion Ni2+
Storage 4–30°C
Dry Ice No
Hazardous No

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Availability:
24/48H Stock Items & 2 to 6 Weeks non Stock Items.

Description

Ni NTA Beads are designed for the purification of 6xHis-tagged proteins expressed using a range of expression systems, including E. coli, yeast, insect cells, and mammalian cells.

The beads consist of 90 µm 4% agarose beads coupled with nitrilotriacetic acid (NTA) and charged with nickel ions (Ni2+). The NTA chelating group forms a stable coordination structure with nickel ions, enabling selective binding of His-tagged proteins.

The bead matrix is compatible with certain concentrations of reducing agents, denaturing agents, detergents, and other commonly used additives, providing flexibility for different protein purification conditions.

Key Features & Benefits

6xHis-Tagged Protein Purification

Designed for affinity purification of recombinant proteins containing a 6xHis tag.

 Ni-NTA Affinity Ligand

Nitrilotriacetic acid is coupled to the agarose matrix and charged with nickel ions for selective protein binding.
4% Agarose Matrix

The 4% agarose bead matrix provides a solid support for affinity chromatography applications.

 90 µm Beads

The 90 µm bead format is suitable for protein purification workflows using affinity chromatography.
Broad Expression System Compatibility

Suitable for purification of proteins expressed in E. coli, yeast, insect cells, and mammalian cells.

 Compatible with Common Additives

Compatible with certain concentrations of reducing agents, denaturing agents, detergents, and other additives.
Stable Nickel Coordination

The NTA-Ni2+ coordination system provides stable interaction for selective capture of His-tagged proteins.

 Wide Applicability

Provides a versatile affinity purification option for recombinant protein research.

Applications

  • Purification of 6xHis-tagged recombinant proteins
  • Affinity chromatography
  • Recombinant protein purification
  • Protein purification from E. coli expression systems
  • Protein purification from yeast expression systems
  • Protein purification from insect cells
  • Protein purification from mammalian cells

Storage Conditions

Storage temperature: 4–30°C

Notes

To be quoted based on destination and order volume.

Ni NTA Beads

Ni-NTA agarose beads for selective purification of 6xHis-tagged proteins.

6th Oct 2026

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